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Research has also indicated that L-carnitine plays a role in boosting the number of testosterone receptors, enhancing nitric oxide production, and may facilitate the action of the anabolic hormone IGF-1

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Additionally, most studies measure AMPK activity via single time-point assessments of p-AMPK Thr172, without quantifying downstream pathway dynamics (e.g., ACC phosphorylation, ULK1 activation) or tissue-specific AMPK isoform expressioncritical for understanding context-dependent regulation in NAFLD

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